General Cell Based Assays
Cell Signaling Technology Glucose-6-Phosphate Dehydrogenase Activity Assay Kit, 100 Assays, 1 Kit
The Glucose-6-Phosphate Dehydrogenase (G6PD) Activity Assay Kit provides a sensitive and simple method for the detection of G6PD activity in various samples. During the assay, G6PD oxidizes glucose-6-phosphate in the presence of NADP+ to generate NADPH, which is subsequently amplified by a diaphorase-cycling system to produce highly fluorescent resorufin molecules. The resulting fluorescence is directly proportional to the G6PD activity in the sample and can be measured using a plate reader at approximately 540 nm excitation and 590 nm emission.
- Rapid, sensitive, and simple detection of enzyme activity
- Designed for use in a 96 well format with 100 assays per kit
- Fluorescent readout proportional to enzyme activity
- Compatible with broad species reactivity
Cell Signaling Technology Active Rho Detection Kit, RhoA, Human/Mouse, 1 Kit
The Active Rho Detection Kit provides all necessary reagents for measuring the activation of Rho GTPase in cells. The system utilizes a GST-Rhotekin-RBD fusion protein to selectively bind the activated, GTP-bound form of Rho. This complex is then isolated via immunoprecipitation with glutathione resin, allowing for the subsequent determination of Rho activation levels through western blot analysis with a specific Rho rabbit antibody.
- Detects endogenous levels of GTP-bound (active) Rho.
- Includes GST-Rhotekin-RBD fusion protein, glutathione resin, Rho rabbit antibody, and essential buffers for 30 immunoprecipitations.
- Validated for use with human and mouse samples.
- Suitable for functional analysis applications.
Cell Signaling Technology XTT Cell Viability Kit, Colorimetric Assay, 1000 Assays
The XTT Cell Viability Kit is a colorimetric assay designed for the detection of cellular metabolic activities. During the assay, the yellow tetrazolium salt XTT is reduced to a highly colored, water-soluble formazan dye by dehydrogenase enzymes present in metabolically active cells. The amount of formazan produced is directly proportional to the number of viable cells in a sample, allowing for quantification by measuring absorbance at 450 nm using a spectrophotometer.
- Does not require radioactive materials, cell fixing, or cell permeabilization, allowing for further analysis of examined cells
- Includes an electron coupling reagent, PMS (N-Methylphenazonium methyl sulfate), to enhance the efficiency of XTT reduction
- Features a formazan dye that is soluble in aqueous solution
- Compatible with high-throughput screening applications
Cell Signaling Technology Cyclic GMP Chemiluminescent Assay Kit, Competition ELISA, 96 Assays, 1 Kit
The Cyclic GMP XP™ Chemiluminescent Assay Kit is a competition enzyme-linked immunoassay designed to determine cGMP levels in cell or tissue samples. The assay utilizes an anti-cGMP XP™ Rabbit mAb immobilized on a 96-well plate to bind cGMP, where sample cGMP competes with a fixed amount of HRP-linked cGMP. Chemiluminescent reagent is subsequently added for signal development, and the resulting light emission in relative light units (RLU) is inversely proportional to the amount of cGMP present in the sample.
- Quantitative analysis of cGMP levels in diverse samples.
- Competitive immunoassay design for precise detection.
- Sensitivity range of 2 nM to 200 nM cGMP.
- Broad species reactivity.
- Minimal cross-reactivity with cIMP and no cross-reactivity with other tested nucleotides.
Cell Signaling Technology Max (S20) Antibody, Rabbit, Polyclonal, Unconjugated, 100 μL
The Max (S20) Antibody is a polyclonal antibody that specifically detects endogenous levels of total Max protein. It is generated by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of the Max protein, a central component of the Myc/Max/Mad transcriptional regulatory network.
- Validated for use in western blotting and immunoprecipitation applications
- Purified by protein A and peptide affinity chromatography
- Recognizes Max protein in human, mouse, and rat species
- Supplied in a formulation containing glycerol, BSA, and sodium HEPES
- Designed for endogenous protein detection
Cell Signaling Technology Akt Pathway IHC Sampler Kit, 1 Kit
The SignalStain™ Akt Pathway IHC Sampler Kit provides a comprehensive solution for examining proteins involved in Akt signaling within paraffin-embedded tissues or cells. Each antibody included in the kit is rigorously validated for use in immunohistochemical assays using multiple approaches. The kit features antibodies detecting endogenous levels of target proteins without cross-reactivity to related proteins, ensuring precise results. It is supplied with control slides to verify assay performance and a primary antibody diluent.
- Validated antibodies for immunohistochemical assays
- Includes control slides for performance verification
- Detects endogenous levels of target proteins
- Supplied with primary antibody diluent
- Suitable for paraffin-embedded tissues or cells
Bioassay Systems EnzyChrom™ Sialic Acid Assay Kit. For quantitative determination of free and total sialic acid (NANA) and evaluation of drug effects on its metabolism.
For quantitative determination of free and total sialic acid (NANA) and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate: 0.02 to 1 mM sialic acid for colorimetric assays and 2 to 100 µM for fluorimetric assays. Simple and convenient. Can detect free sialic acid by addition of a single working reagent and incubation for 60 min at room temperature or total sialic acid by pre-treating samples with a 60 min hydrolysis step. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 2 hrs. Kit size: 100 tests. Detection limit: OD, FL: 20, 2 µM.
Abcam Hemoglobin Assay Kit (Colorimetric)
Hemoglobin Assay Kit (Colorimetric)
The product is subject to the following: Abcam Restricted Use Statement
Abcam Hydrogen Peroxide Assay Kit
Hydrogen Peroxide Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-NF-κB (Ser536) Detection Kit, 500 Assay Points
HTRF Human & Mouse Phospho-NF-κB (Ser536) Detection Kit, 500 Assay Points
Eurofins Discoverx AssayComplete Freezing Reagent F2, 2 x 50. (92-5102FR)
AssayComplete Freezing Reagent F2 is an optimized freezing reagent for cryopreservation of Eurofins DiscoverX cell lines. This reagent ensures optimal cell viability and assay performance upon thawing. This product is provided as 2 bottles of 50 mL reagent each. Please refer to cell line specific datasheet to determine the recommended freezing reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.